Biomembrane Protocols: I. Isolation and Analysis
Editor(s): John M. Graham1, Joan A. Higgins2
Affiliation(s): (1),Merseyside Innovation Centre, Liverpool UK (2)Department of Molecular Biology and Biotechnology,University of Sheffield, UK
Series: Methods in Molecular Biology Volume No.: 19
Print ISBN: 978-0-89603-236-1

Info:

Editorial Reviews
Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component(organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. Subcellular Fractionation contains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

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